caproBox™ for reproducible and comparable UV cross-linking results

 

Constant cooling during photo cross-linking prevents protein damages

The success of photo cross-linking is dependent on several parameters, such as time of irradiation, wavelength, distance to sample, temperature. The caproBox™ is developed to enable temperature controlled UV photo cross-linking reactions. The instrument combines timed UV irradiation and temperature control in one system and allows a convenient, safe, and reproducible photo cross-linking in small reaction volumes.

The following features make it ideally suited for photo cross-linking processes:

• Up to 96 samples can be irradiated simultaneously
• Precise temperature control of the samples within 0 - 4 °C
• Programmable irradiation time
• Automatic shut down of UV irradiation when protection cover is opened
• Benchtop, space saving design
• Easy and intuitive to operate

The caproBox is mainly used for cross-linking of affinity bound proteins to Capture Compounds™. During this process, the photoreactivity group of the Capture Compound is activated and covalently attached to the targeted proteins. Constant cooling of the samples prevents denaturation of proteins and permits a gentle UV irradiation during all photo cross-linking reactions.

However, the caproBox may also be used for applications outside of the CCMS technology - whenever simultaneous cooling and UV irradiation are needed (e.g. photo-reactive amino acids).

The CCMS Starter Kit allows you an easy and convinient implementation of CCMS technology in your lab.

The CCMS Starter Kit contains a caproBox™ for incubating and UV cross-linking the Capture Compound™ and the prey proteins, three caproKits™ of your choice, a caproMag™ for sample handling and a Biotin Capping Kit.

Please feel free to contact us for using the caproBox™ for applications outside of the CCMS technology.

Please email: info(at)caprotec.com