The SAH caproKit^™ is a complete system to investigate SAM / SAH binding proteins.

SAM is the major source of methyl groups in biological reactions and used by methyltransferases in many important physiological processes. For example, DNA/RNA and Histone methylation provide important mechanisms for the regulation of genetic activity, specific methylation sites mediate restriction enzyme resistance, the degradation of neurotransmitters as well as biosynthesis and degradation and de-toxification of many compounds is accompanied through the transfer of methylgroups.

S-adenosyl-L-homocysteine (SAH) has a similar K_d as SAM and is used as selectivity function instead of SAM, due to higher stability. The Capture Compound^™ scaffold is attached to the N6 position of SAH, as many MTases tolerate modifications at this position (1).

The Kit can be used to discover and profile SAM binding proteins from a variety of different sources. Downstream of the CCMS process, the proteins can be subjected to tryptic digests, MS or gel electrophoresis (2).

 

1) G. Pljevaljcic, F. Schmidt, E. Weinhold, (2004) Sequence-specific methyltransferase-induced labeling of DNA (SMILing DNA); ChemBioChem (5); 265-269.

2) H. Köster et al, (2007) Capture Compound Mass Spectrometry: A Technology for the Investigation of Small Molecule Protein Interactions; Assay and Drug Development Tech. (5); 381-390.