Enhanced functional isolation of phosphodiesterases using the C2-cAMP Capture Compound
The C8-cAMP Capture Compound™ attached to the Capture Compound scaffold via the C8 position of the cyclic adenosyl-monophosphate (cAMP) effectively captures regulatory subunits of the Protein Kinase A (PKA) and cAMP-regulated hyperpolarisation and cyclic nucleotide-gated (HCN) ion channel.
Using cAMP attached at the C2 position to the Capture Compound scaffold results in a complementary profile of cAMP-binding proteins, especially including captured phosphodiesterases in the OnBead configuration.
The C2-cAMP caproKit™ includes the C2-cAMP Capture Compound, cAMP competitor, all buffers, positive control protein, and streptavidin magnetic beads.
For profiling the whole cAMP-interacting proteome we recommend to use the cAMP caproKit™ complete.
Please note, that CCMS reagents (caproKits™) will perform best when used with the optimized equipment (caproBox™ and caproMag™).
For the most reproducible results it is recommended to standardize parameters such as UV wavelength, distance between sample and light source, temperature and incubation time. This can best be accomplished using a caproBox™ for cross-linking.
If you are using the proprietary CCMS technology for the first time, caprotec offers a convenient CCMS Starter Kit including a free choice of 3 independent caproKits™, Biotin Capping Kit, caproBox™, and caproMag™. Please feel free to contact us for any additional questions related to using caprotec products!
caproKits allow for discovering, isolating, and profiling members of functional protein families within a variety of biological samples. Downstream of this process the proteins can be subjected to tryptic digestion, MS or gel electrophoresis.