The GDP caproKit is most suitable for the functional isolation of small GTPases
The functional isolation of proteome subsets based on small molecule-protein interactions is an increasingly popular field in functional proteomics (1). Entire protein families may be profiled on the basis of their common interaction with a metabolite or small molecule inhibitor. This is enabled by novel multifunctional small molecule probes. One innovative approach in this field are Capture Compounds™ that contain a small molecule of interest, e.g. guanosine diphosphate (GDP) to bind target proteins, a photo-inducible reactivity function for the covalent attachment of bound proteins, and a sorting function to isolate Capture Compound-protein conjugates from complex biological samples for the direct identification (2, 3).
Guanosine nucleotide binding proteins, G proteins are involved in second messenger cascades and function as molecular switches. Alternating between an inactive guanosine diphosphate (GDP) and active guanosine triphosphate (GTP) bound state to regulate downstream cell processes.
G proteins can be separated into two families: the heterotrimeric G proteins which are activated by GPCRs and consist of alpha, beta, and gamma subunits as well as the monomeric small G proteins which belong to the Ras superfamily of small GTPases. These proteins are homologous to the alpha subunit of heterotrimeric G proteins. More than thousands of G protein-coupled receptors are encoded by the human genome (4). Among them roughly 150 have unknown functions.
The GDP caproKit™ will be a valuable tool for the proteomic profiling of the small G protein superfamily.
The GDP caproKit™ includes the GDP Capture Compound, GDP competitor, all buffers, positive control protein, and streptavidin magnetic beads.
Please note, that CCMS reagents (caproKits™) will perform best when used with the optimized equipment (caproBox™ and caproMag™).
For the most reproducible results it is recommended to standardize parameters such as UV wavelength, distance between sample and light source, temperature and incubation time. This can best be accomplished using a caproBox™ for cross-linking.
If you are using the proprietary CCMS technology for the first time, caprotec offers a convenient CCMS Starter Kit including a free choice of 3 independent caproKits™, Biotin Capping Kit, caproBox™, and caproMag™. Please feel free to contact us for any additional questions related to using caprotec products!
