Cyclic guanosine monophosphate (cGMP) is an important mediator for the activation of signaling cascades
Knowledge about cGMP-binding proteins is important to signal transduction involved in the activation of protein kinases
Cyclic guanosine monophosphate (cGMP) is synthesized by the guanylatcyclase using GTP as substrate. cGMP is an important second messanger for the activation of signaling cascades. It is involved e.g. in processes of hormone- and neurotransmitter-dependent signaling and seeing or chemotaxis of spermatozoon. The activation of many specific signaling cascades, which are not completely understood, occur via cGMP-dependent protein kinases.
New studies revealed an important role of the cGMP pathway in ovarian functions, including steroidogenesis, ovulation, and granulosa cell proliferation (1). Additionally, there is evidence for a leation of cAMP and cGMP to nitric oxide synthase isoforms and vasoactive intestinal polypeptide-containing nerves (VIP). Nitric oxide and VIP mediate biological signals through cGMP and cAMP (2).
To aid the understanding of these processes, caprotec has developed the N²-cGMP caproKit™ to isolate and identify cGMP-binding proteins.
The N²-cGMP caproKit™ includes the N²-cGMP Capture Compound, cGMP competitor, all buffers, positive control protein, and streptavidin magnetic beads.
caprotec offers additional cGMP Capture Compounds on request (C8 and 2´O). Please feel free to contact us for detailed information at info(at)caprotec.com
The respective cGMP and cAMP caproKits can be used in combination to increase the understanding of the time-dependent interplay of the different biochemical and signaling pathways, or the structure and function of all cellular protein. Especially protein modifications and differences in the level of protein expression are involved in the development of certain disease states (3, 4, 5) and constitue an additional layer of complexity. The cNMP caproKits aid in the identification of proteins which belong to signal transduction interactome.
Please note, that CCMS reagents (caproKits™) will perform best when used with the optimized equipment (caproBox™ and caproMag™).
For the most reproducible results it is recommended to standardize parameters such as UV wavelength, distance between sample and light source, temperature and incubation time. This can best be accomplished using a caproBox™ for cross-linking.
If you are using the proprietary CCMS technology for the first time, caprotec offers a convenient CCMS Starter Kit including a free choice of 3 independent caproKits™, Biotin Capping Kit, caproBox™, and caproMag™. Please feel free to contact us for any additional questions related to using caprotec products!
References:
1) Sasseville, M., Côté, N., Gagnon, M.C., Richard, F.J., Endocrinology 2008, 149, 5568-5576
