The Stauro caproKit™ represents an ideal kinase profiling system to improve and support drug discovery projects.
Protein kinases play a key role in the regulation of signaling cascades e.g. for cell proliferation, apotosis, or differentiation. Disruption in the regulation of these inter-connected networks are closely associated with diseases like neuronal disorders, cancer, or inflammatory reactions. As many protein kinases are proto oncoproteins or involved in oncogenic signaling a major component towards cancer treatment is the understanding interplay of different signaling cascades to aid in the development of effective therapies.
Staurosporine is a natural product which was originally isolated from the bacterium Streptomyces staurosporeus (1). The interest in this alkaloid resulted from its anti-fungal and anti-hypertensive properties (2). Staurosporine is a potent inhibitor for a large number of serine/threonine kinases, like protein kinase C (PKC), cAMP- and cGMP-dependent protein kinases and also suppresses several tyrosine kinases, like p60v-src, EGF-, or insulin receptor. It binds to many kinases with a preference to ATP-binding proteins with a high affinity but with little selectivity (3). This lack of selectivity has made staurosporine a valuable tool to understand the time-dependent interplay of the different biochemical and signalling pathways, or the structure and function of cellular proteins.
Staurosporine is attached to the Capture Compound™ scaffold and used as selectivity function for the functional isolation of protein kinases and support future drug discovery approaches.
The Stauro caproKit can be used to discover and profile protein kinases from a variety of different sources.
