Advantages of CCMS compared to other methods
Capture Compounds™ are small molecules, thus their integrity can be unambiguously assessed by analytical methods. A coupling of ligands or proteins to a solid support is not necessary.
The Capture Compound™ protein interaction is taking place in a homogeneous solution, no solid phase interactions are required during the critical affinity interaction to capture protein targets, no bias with regards to protein size is created.
Capture Compounds™ can be modified to react in hydrophilic and lipophilic environments, allowing to investigate a wide range of target proteins.
Weak and strong interacting proteins can be identified by varying the ratio of Capture Compound™ to protein. Stringent wash conditions can be employed even with low affinity binders due to the covalent photo cross-linking.
Download Advantages of Capture Compounds Mass Spectrometry in comparison of bifunctional molecules, affinity chromatography and immune precipitation (2.8 MB, high resolution).