Photo Cross-linking
The advantage of CCMS is the covalent attachment of the Capture Compound to the target proteins via UV light.
This second step is performed through photo cross-linking the reactivity function (aromatic azides or -diazirines) with a short UV flash to the proteins. This is accomplished using the caproBox™, a combination of UV-irradiation and cooling device. The success of photo cross-linking is dependent on several parameters (such as time, wavelength, distance to sample, temperature). The caproBox™ controlls these parameters and guarantees reproducible and comparable results. The functional selectivity of the capture reaction can easily be determined by a control experiment in the presence of molecules which compete with the selectivity function.
- Photo cross-linking generates a covalent bond between Capture Compound and target protein
Click the slides to zoom in for detailed information of CCMS process
in a structural or in a schematic view.
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Usually, the photo cross-linking site is in close spatial proximity to the binding site mediated by the selectivity function; this knowledge may reveal initial information in locating unknown binding sites.
