Photo Cross-linking
The advantage of CCMS is the covalent attachment of the Capture Compound to the target proteins via UV light.
This second step is performed through photo cross-linking the reactivity function (aromatic azides or -diazirines) with a short UV flash to the proteins. This is accomplished using the caproBox™, a combination of UV-irradiation and cooling device. The functional selectivity of the capture reaction can easily be determined by a control experiment in the presence of molecules which compete with the selectivity function.
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- Photo cross-linking generates a covalent bond between Capture Compound and target protein
Usually, the photo cross-linking site is in close spatial proximity to the binding site mediated by the selectivity function; this knowledge may reveal initial information in locating unknown binding sites.
