Protein Isolation in Solution
Biotin is used as sorting function to isolate captured proteins.
The third step uses streptavidin-coated magnetic particles to isolate the Capture CompoundTM - protein complex from the biological sample. This step does not depend on a solid phase interaction. Following removal of the supernatant, the proteins can be analyzed further by electrophoresis and / or mass spectrometry including enzymatic digest and sequencing of the captured peptides.
Click the slides to zoom in for detailed information of CCMS process
in a structural or in a schematic view.
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A very important aspect is that CCMS enables the investigation and isolation of lipophilic proteins, e.g membrane associated proteins, which are difficult or impossible to analyze with other methods.
Capture Compounds enable an efficient complexity reduction of the proteome to a subset of proteins based on their affinity to the selectivity group. Thus, CCMS allows discovering, isolating and profiling of functional protein families within a large variety of biological samples.