Protein samples from different sources and origins can be the starting point of a CCMS experiment.
Material from cell culture, bacteria, plants and various tissues can be investigated and in all these cases, CCMS offers a universal solution to address a selected sub-population of the proteome. Contrary to many other technologies, depletion of albumin or other highly abundant proteins is not necessary during the preparation step due to the specificity of the protein Capture Compoundô interaction. Additionally, target molecules do not need to be active enzymes since the assay is not dependent on enzyme activity and substrate conversion; the capture event is strictly based on reversible affinity interaction.
In the first step, Capture Compounds are incubated with a biological sample. In this phase a reversible, affinity driven interaction between the selectivity function and the target proteins occurs.